Molecular Microbiology Laboratory

Molecular Microbiology Laboratory PDF
Author: Walt Ream
Publisher: Academic Press
ISBN: 0123977525
Size: 23.23 MB
Format: PDF
Category : Science
Languages : en
Pages : 259
View: 6876

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Book Description: Molecular Microbiology Laboratory, Second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles. Exposes students to the new molecular-based techniques Provides faculty with an authoritative, accessible resource for teaching protocols The only manual to incorporate writing exercises, presentation skills and tools for reading primary literature into the curriculum Based on a successful course for which the author won a teaching award New to this Edition: - Presents a real-world study of bacterial populations in the environment in the final experiment - Provides an overview of molecular biology in a new review chapter - Demonstrates how to design an experiment and how to interpret the results - Covers grant proposal writing and how panels review proposals - Presents guidance on public speaking and preparing PowerPoint presentations - Includes tutorials on three widely used software packages

Advanced Techniques In Diagnostic Microbiology

Advanced Techniques in Diagnostic Microbiology PDF
Author: Yi-Wei Tang
Publisher: Springer Science & Business Media
ISBN: 1461439698
Size: 46.26 MB
Format: PDF, Kindle
Category : Medical
Languages : en
Pages : 957
View: 7370

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Book Description: In the United States, hospitals annually report over 5 million cases of infectious-disease-related illnesses: clinical microbiology laboratories in these hospitals are engaged in detecting and identifying the pathogenic microorganisms in clinical specimens collected from these patients with suspected infections. Clearly, the timely and accurate detection/identification of these microbial pathogens is critical for patient treatment decisions and outcomes for millions of patients each year. Despite an appreciation that the outcome of an infectious-disease-related illness is directly related to the time required to detect and identify a microbial pathogen, clinical microbiology laboratories in the United States as well as worldwide have long been hampered by traditional culture-based assays, which may require prolonged incubation time for slowly growing microorganisms such as Mycobacterium tuberculosis. Moreover, traditional culture-based assays often require multiple steps with additional time needed for discernment of species and/or detection of antimicrobial resistance. Finally, these traditional, slow multistep culture-based assays are labor-intensive and required skilled clinical microbiologists at the bench. Over the past several decades, advanced molecular techniques in diagnostic microbiology quietly have been revolutionizing the practice of clinical microbiology in the hospital setting. Indeed, molecular diagnostic testing in general and nucleic-acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. There is no question that the development of rapid molecular techniques for nucleic acid amplification/characterization combined with automation and user-friendly software has greatly broadened the diagnostic capabilities of the clinical microbiology laboratory. These technical advances in molecular microbiology over the first decade of the 21st Century have profoundly influenced the physical structure of clinical microbiology laboratories as well as their staffing patterns, workflow, and turnaround time. These molecular microbiology advances have also resulted in the need for a revised and updated second edition of Advanced Techniques in Diagnostic Microbiology. This second edition again provides an updated and comprehensive description of the ongoing evolution of molecular methods for the diagnosis of infectious diseases. In addition, many new chapters have been added, including a chapter on the clinical interpretation and relevance of advanced technique results. The second edition, like the first edition, includes both a “techniques” section describing the latest molecular techniques and an “applications” section describing how these advanced molecular techniques are being used in the clinical setting. Finally, the second edition, like the first edition, utilizes a diverse team of authors who have compiled chapters that provide the reader with comprehensive and useable information on advanced molecular microbiology techniques.

Pcr For Clinical Microbiology

PCR for Clinical Microbiology PDF
Author: Ian W.J. Carter
Publisher: Springer Science & Business Media
ISBN: 9789048190393
Size: 21.41 MB
Format: PDF, ePub, Mobi
Category : Science
Languages : en
Pages : 438
View: 7204

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Book Description: Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology

Nucleic Acid Testing For Human Disease

Nucleic Acid Testing for Human Disease PDF
Author: Attila Lorincz
Publisher: CRC Press
ISBN: 1000713431
Size: 73.20 MB
Format: PDF, ePub
Category : Medical
Languages : en
Pages : 488
View: 7069

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Book Description: Nucleic Acid Testing for Human Disease describes various techniques including target and signal amplification-based NAT procedures, microarrays, bead-based multiplex assays, in situ hybridization, and SNP techniques. This book discusses RNA expression profiling and laboratory issues such as the need for proper validation of tests intended fo

Methods For General And Molecular Microbiology

Methods for General and Molecular Microbiology PDF
Author: C. A. Reddy
Publisher: American Society for Microbiology Press
ISBN: 1555812236
Size: 37.35 MB
Format: PDF, Mobi
Category : Science
Languages : en
Pages : 1069
View: 6006

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Book Description: A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.

Journal Of Molecular Microbiology And Biotechnology

Journal of Molecular Microbiology and Biotechnology PDF
Author:
Publisher:
ISBN:
Size: 51.13 MB
Format: PDF, Mobi
Category : Biotechnology
Languages : en
Pages :
View: 7502

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Book Description:

Molecular Pathology An Issue Of Clinics In Laboratory Medicine

Molecular Pathology  An Issue of Clinics in Laboratory Medicine  PDF
Author: Martin H. Bluth
Publisher: Elsevier Health Sciences
ISBN: 0323261051
Size: 20.57 MB
Format: PDF
Category : Medical
Languages : en
Pages : 209
View: 2639

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Book Description: Molecular pathology has significantly matured over the past decade to establish itself as a discipline in its own right. Patient diagnosis, prognosis, management and care have been influenced by molecular pathology testing algorithms on a global scale. Whether it pertains to carrier status of deleterious genes, diagnosis by molecular techniques, assessment of appropriate remission post treatment, parentage or forensics testing – molecular pathology continues to increase its presence and influence in the diagnostic laboratory armamentarium. Furthermore, intellectualization and proprietization of molecular pathology with respect to the discovery of new genes and or new techniques continues to pose new challenges of test ownership, legalities, liabilities, widespread acceptance and utility. This issue of Laboratory Clinics seeks to provide selective state of the art understanding of molecular pathology with respect to its relationship to key pathology disciplines as well as the current challenges and promise for the future.

Introduction To Diagnostic Microbiology For The Laboratory Sciences

Introduction to Diagnostic Microbiology for the Laboratory Sciences PDF
Author: Maria Dannessa Delost
Publisher: Jones & Bartlett Publishers
ISBN: 1284032329
Size: 12.63 MB
Format: PDF, Kindle
Category : Medical
Languages : en
Pages : 588
View: 1386

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Book Description: Introduction to Diagnostic Microbiology for the Laboratory Sciences provides a foundation in microbiology that is essential for a career as a medical laboratory technologist/technician (MLT). A key text for students and a helpful reference for practitioners, it reviews the microorganisms most commonly encountered in clinical settings and clearly explains basic laboratory procedures. This text provides a concise overview of topics and facilitates comprehension with learning objectives, key terms, case studies, and review questions. In addition, the text includes laboratory exercises, eliminating the need for a separate laboratory manual. Covering content required in the MLT curriculum and featured on the certification exam, this accessible text will help prepare students for a career in laboratory science. Key Features • Reviews the microorganisms most important in clinical practice • Explains basic laboratory procedures, such as specimen collection and staining • Includes laboratory exercises in the text–no need for a separate manual • Serves as a helpful on-the-job reference for laboratory practitioners • Provides practice questions to help students prepare for the medical technology certification exam CHAPTER PEDAGOGY: Chapter Outline, Key Terms, Learning Objectives, Procedures, Laboratory Exercises, Case Studies, Review Questions INSTRUCTOR RESOURCES: Image Bank with 247 photos and illustrations; PowerPoint Presentations per chapter; Laboratory Exercise Worksheets; and a Test Bank with 450 multiple choice questions and a 225-question exam. Introduction to Diagnostic Microbiology for the Laboratory Sciences is on the recommended reading list to prepare for the ASCP MLT exam. (American Society for Clinical Pathology, Medical Laboratory Technician exam)

Rna Tumor Viruses Oncogenes Human Cancer And Aids On The Frontiers Of Understanding

RNA Tumor Viruses  Oncogenes  Human Cancer and AIDS  On the Frontiers of Understanding PDF
Author: Philip Furmanski
Publisher: Springer Science & Business Media
ISBN: 1461325838
Size: 42.71 MB
Format: PDF, ePub
Category : Medical
Languages : en
Pages : 410
View: 5280

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Book Description: We stand today on the threshold of a new understanding of cancer. Primarily through the powerful tools of molecular biology, unified hypotheses explaining the origins of the disease are emerging and rapidly being validated. This volume, which presents the latest findings from laboratories throughout the world on the role of RNA tumor viruses in cancer, is a celebration of these achievements and a prediction of further progress leading ultimately to the control of the disease. It is important in this context to recall the natural history or life cycle of RNA cancer virology. From the earliest days of the science, when viruses were first recognized as distinct biologic agents of etiologic significance, their role in cancer was proposed and hotly debated. The critical early discoveries, even those made as recently as 25 years ago, were met with rejection; not skepticism or cautious restraint, but outright rejection. During the 60's, there was a gradual acceptance of the association between viruses and cancer, the result of landmark studies in experimental systems, and this led to a frenzy of activity in the field. There followed another period of doubt and uncertainty, due to the difficulty in attempting to apply directly, and in retrospect inappropriately, the tenets of infectious disease to human cancers, only to have the field resurrected, revitalized and redirected by the explosion of progress in molecular biology and genetics.

Molecular Medical Microbiology

Molecular Medical Microbiology PDF
Author: Yi-Wei Tang
Publisher: Academic Press
ISBN: 0123977630
Size: 48.65 MB
Format: PDF, Docs
Category : Science
Languages : en
Pages : 2216
View: 2020

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Book Description: The molecular age has brought about dramatic changes in medical microbiology, and great leaps in our understanding of the mechanisms of infectious disease. Molecular Medical Microbiology is the first book to synthesise the many new developments in both molecular and clinical research in a single comprehensive resource. This timely and authoritative three-volume work is an invaluable reference source of medical bacteriology. Comprising more than 100 chapters, organized into 17 major sections, the scope of this impressive work is wide-ranging. Written by experts in the field, chapters include cutting-edge information, and clinical overviews for each major bacterial group, in addition to the latest updates on vaccine development, molecular technology and diagnostic technology. Topics covered include bacterial structure, cell function, and genetics; mechanisms of pathogenesis and prevention; antibacterial agents; and infections ranging from gastrointestinal to urinary tract, centrtal nervous system, respiratory tract, and more. The first comprehensive and accessible reference on molecular medical microbiology Full color presentation througout In-depth discussion of individual pathogenic bacteria in a system-oriented approach Includes a clinical overview for each major bacterial group Presents the latest information on vaccine development, molecular technology, and diagnostic technology More than 100 chapters covering all major groups of bacteria Written by an international panel of authors who are experts in their respective disciplines